2-dimensional gel electrophoresis reloaded

Two-Dimensional Gel Electrophoresis (2-DE)

Two-dimensional gel electrophoresis (2-DE) is able to separate hundreds to thousands of proteins or polypeptides by coupling IsoElectric Focusing (IEF) in first dimension and Sodium Dodecyl Sulphate PolyAcrylamide-Gel Electrophoresis (SDS-PAGE) in second dimension. This particular configuration is called classical 2-DE: IEF separates proteins in function of their isoelectric point (pI) and SDS-...

Two-dimensional Gel Electrophoresis

Identification by Two-Dimensional Gel Electrophoresis

We have previously identified proteins in fractions of culture filtrate ofMycobacterium tuberculosis with the capacity to induce cytokine production in monocytes, by using a technique we defined as "monocyte Western blotting" (immunoblotting). In this series of experiments, we have extended this technique to two-dimensional gel electrophoresis and have identified a novel 58-kDa protein ofM. tub...

Two-dimensional gel electrophoresis: an overview

Two-Dimensional Gel Electrophoresis Image Registration

Two-dimensional gel electrophoresis (2DGE) is the leading technique to separate individual proteins in biological samples with many biological and pharmaceutical applications, e.g., drug development. The technique results in an image in which the proteins appear as dark spots on a bright background. The analysis of these images is very time consuming and requires a large amount of manual work. ...